Academic journal article Iranian Journal of Public Health

EVALUATION OF CURING AGENTS IN PSEUDOMONAS AERUGINOSA ISOLATES FROM BURNED PATIENTS IN ISFAHAN, Iran

Academic journal article Iranian Journal of Public Health

EVALUATION OF CURING AGENTS IN PSEUDOMONAS AERUGINOSA ISOLATES FROM BURNED PATIENTS IN ISFAHAN, Iran

Article excerpt

Background: The present study was conducted to evaluate the antibiotic resistant occurrence of plasmid and to compare the curing agents in Pseudomonas aeruginosa isolated from wound of burned patients.

Methods: 220 clinical samples were collected from hospital- ized burned patients in Isfahan Burn Hospital within three months (Mar 2012 to Jun 2012). Identification of Pseudomonas aeruginosa was performed using standard biochemical tests and then it was confirmed with API 20NE Systems. Antibi- otic resistant was determined by standard Agar disk diffusion against three groups of antibiotics viz., meropenem, ceftriax- on, ciprofloxacin. Then plasmid profile was detected by plasmid spin column extraction method, respectively. Fur- thermore, Plasmid curing was done on this strain using eth- idium bromide, rifampin and the elevated temperature.

Results: The results obtained from this study indicated that 60 Pseudomonas aeruginosa isolates were resistant to three anti- biotics tested. The results showed that the frequency of plasmid curing induced by ethidium bromide was 60%, while high temperature (44°C) and rifampicin couldn not cure the isolated strain. The isolates lost their plasmid after treating with ethidium bromide at 1000 and 2000µg/ml. …

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