Academic journal article Iranian Journal of Public Health

MUTATION PATTERN EVALUATION OF HUMAN CYTOMEGALOVIRUS (HCMV) FOR RESISTANCE TO GANCICLOVIR IN Iran IMMUNO-SUPPRESSED PATIENTS DURING 2012-2013

Academic journal article Iranian Journal of Public Health

MUTATION PATTERN EVALUATION OF HUMAN CYTOMEGALOVIRUS (HCMV) FOR RESISTANCE TO GANCICLOVIR IN Iran IMMUNO-SUPPRESSED PATIENTS DURING 2012-2013

Article excerpt

Background: Human cytomegalovirus (HCMV) remains as an important pathogen for immuno-compromised individu- als. Ganciclovir is one of the best drugs used for treatment of this disease, whereas prolonged therapy can cause HCMV resistance to ganciclovir. The main reasons for resistance to ganciclovir drug are mutations in ul97 gene of HCMV.The goal of the study was to evaluate the mutation pattern of HCMV to ganciclovir in immuno-suppressed patients before consuming antiviral medications especially ganciclovir.

Methods: 128 positive clinical HCMV isolated samples for immuno-suppressed patients were selected. 939 bp and 679 bp of ul97 gene were amplified with specific nested PCR primers. The primers were targeted at the highly conserved regions of ul97 gene between the codon numbers of 405 - 607. These codons were correlated to ganciclovir resistance. PCR products of all samples were sequenced. CLC sequence viewer software was employed for analysis of all specimens. …

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