Academic journal article Iranian Journal of Public Health

THE FREQUNCY OF EXTENDED-SPECTRUM BETA-LACTAMASE-PRODUCING ESCHERICHIA COLI AT AN EDUCATIONAL HOSPITAL IN SHAHREKORD, Iran

Academic journal article Iranian Journal of Public Health

THE FREQUNCY OF EXTENDED-SPECTRUM BETA-LACTAMASE-PRODUCING ESCHERICHIA COLI AT AN EDUCATIONAL HOSPITAL IN SHAHREKORD, Iran

Article excerpt

Background: The current study showed the prevalence of extended-spectrum beta-lactamase-producing Escherichia coli uropathogens in Shahrekord, Iran.

Methods: A total of 200 urinary tract infections (UTIs) of E. coli were collected from an educational hospital in shahrekord, Iran. Uropathogens were identified through cul- ture, microscopy and biochemical tests. The agar disc diffu- sion assay was used to determine the antimicrobial suscepti- bilities of uropathogens. The ESBL positive strains were fur- ther subjected to phenotypic confirmatory tests using sensi- tivity discs, which contained third-generation cephalosporins both with and without clavulanic acid. The discs (ceftazidime (30 µg), ceftazidime + clavulanic acid (30 µg + 10 µg). ex- tended-spectrum beta-lactamase resistance gene VEB were examined in ESBL positive strains using by PCR.

Results: Combined double disc synergy test was applied to detect ESBL in 90 E. …

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