Academic journal article Iranian Journal of Public Health

PREVALENCE OF OXA-1 TYPE ESBLS AMONG CLINICAL ISOLATES OF ENTEROBACTER CLOACAE COLLECTED FROM QAZVIN HOSPITALS, Iran

Academic journal article Iranian Journal of Public Health

PREVALENCE OF OXA-1 TYPE ESBLS AMONG CLINICAL ISOLATES OF ENTEROBACTER CLOACAE COLLECTED FROM QAZVIN HOSPITALS, Iran

Article excerpt

Background: The aim of this study was to determine the frequency of OXA-types among ESBL-producing E. cloacae isolates collected from Qazvin hospitals.

Methods: Totally, 61 ESBL-producing E. cloacae isolates were collected from different clinical samples. Species` iden- tification was performed using standard laboratory methods. ESBL production was confirmed by phenotypic combined disk method as recommended by the Clinical and Laboratory Standards Institute (CLSI). PCR and sequencing were per- formed for detection of blaOXA-1, blaOXA-2, blaOXA-9 and blaOXA-10 genes using specific primers.

Results: In total, 34(55.7%) of ESBL-producing isolates were positive for the presence of blaOXA-1 genes. blaOXA- 2, blaOXA-10 and blaOXA-9 genes were not detected in this study. All blaOXA-1 -producing isolates showed multidrug resistant pattern. …

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