Academic journal article Iranian Journal of Public Health

The Prevalence and Distribution of Aada1, Dhfra1-Aada1 and Dfra1 Gene Cassettes of Class 2 Integron among Clinical Isolates of Staphylococcus Aureus from Hospitals in Tehran: The First Report

Academic journal article Iranian Journal of Public Health

The Prevalence and Distribution of Aada1, Dhfra1-Aada1 and Dfra1 Gene Cassettes of Class 2 Integron among Clinical Isolates of Staphylococcus Aureus from Hospitals in Tehran: The First Report

Article excerpt

Background: The aims of this study were to determine an- timicrobial susceptibility, to detect class 2 integrons in iso- lates of Staphylococcus aureus and to analyze genes cassettes content of class 2 integrons in selected multi-drug resistance (MDR) strains.

Methods: 139 S.aureus isolates were collected from hospitals in Tehran. Antimicrobial susceptibility testing (Streptomycin, Tetracycline, Sulfamethoxazole-Trimethoprim, Gentamicin, Vancomycin, Oxacillin, Levofloxacin, Erythromycin, Spec- tinomycin, Amoxicillin, Ciprofloxacin) was done for MDR isolates by disk diffusion method (Kirby-bauer). E. coli ATCC 25922 was used as the quality control organism in antibio- gram test. The DNA was extracted with phenol-chloroform and class 2 integrase (intI2) genes were detected by PCR. intI2-positive isolates were further analysed for the presence of resistance gene cassettes using specific primers 5'cs/3'cs.

Results: Among 139 S.aureus isolates, 109 (78.4%) strains were considered as MDR. PCR assays (dintI2 gene and inter- nal variable regions (IVRs) of class 2 integron in 49/139 (35. …

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