Academic journal article Iranian Journal of Public Health

Repetitive Element Pcr Fingerprinting (Rep-Pcr) in Klebsiella Pneumoniae Producing Ctx-M in Sanandaj

Academic journal article Iranian Journal of Public Health

Repetitive Element Pcr Fingerprinting (Rep-Pcr) in Klebsiella Pneumoniae Producing Ctx-M in Sanandaj

Article excerpt

Background: Organisms producing CTX-M β-lactamases are emerging as a source of resistance to oxyiminocephalo- sporins such as ceftriaxone and ceftazidime. However, the laboratory detection of these strains is not well defined. The aim of this study was to determine the prevalence of CTX-M and associated risk factors for community-acquired Klebsiella pneumoniae in Sanandaj, Iran.

Methods: In this study, 100 Klebsiella pneumonia strains com- munity-acquired were used. The pattern of antimicrobial re- sistance was determined by double disk diffusion method. The ESBL production was determined by combination disk method using disks containing Cefepime, Cefpodoxime, ceftazidime and cefotaxim alone and in combination with Clavulanic acid. CTX-M type of ESBL producing genes were detected by PCR. A possible clonal relationship among the strains was determined by repetitive extragenic palindromic sequence PCR.

Results: Confirmatory phenotypic test showed that 88% of the strains were ESBL positive. PCR used for the detection of CTX-M gene, showed that 37(42.04%) out of 88 isolates contained such gene. Base on rep-PCR, 31 genotypes among 37 CTX-M-positive samples were detected. …

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