Academic journal article Iranian Journal of Public Health

Determination the Frequency of Aders Genes among Clinical Isolates of Carbapenem Resistance A. Baumannii Strains

Academic journal article Iranian Journal of Public Health

Determination the Frequency of Aders Genes among Clinical Isolates of Carbapenem Resistance A. Baumannii Strains

Article excerpt

Background: Carbapenems are considered as the key treat- ment in many cases of nosocomial infections of Acinetobacter baumannii. AdeABC efflux pump is the first characterized resistance-nodulation-cell division (RND) systems in re- sistant A. baumannii and tightly is regulated by the two- component system AdeS /R. So, the aim of this study was to determine the frequency of adeS and adeR genes among car- bapenem resistant A. baumannii clinical isolates.

Methods: 60 clinical carbapenem resistant A. baumannii iso- lates were obtained from Taleghani and Motahari hospitals in Tehran during 2013. Antimicrobial susceptibility testing was done by Kirby-Bauer method and MIC of imipenem was determined by the microdilution method, according to CLSI 2012. Simultaneously, A. baumannii ATCC27853 was evaluat- ed as the reference strain. DNA extraction was done by boil- ing method and the frequency of the above genes was detect- ed by PCR.

Results: 34 out of 60 A. baumanni strains were isolated from burn wounds of Motahari hospital and 19 from trachea(13), blood(6) of ICU as the frequent ward of Taleghani hospital. Based on the antibiotic resistance test, the most resistance rates were detected to Ceftriaxone, Cefotaxime, Ciprofloxa- cin, Trimethoprim-sulfamethoxaxole, Ceftazidime, Piperacil- lin, Piperacillin-tazobactam (100%) and all isolates (100%) were sensitive to colistin. …

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