Academic journal article Iranian Journal of Public Health

Antimicrobial Resistance and Class 1 Integron in Escherichia Coli Strains Isolated from Different Animal Sources

Academic journal article Iranian Journal of Public Health

Antimicrobial Resistance and Class 1 Integron in Escherichia Coli Strains Isolated from Different Animal Sources

Article excerpt

Background: The aim of the current study was to evaluate the prevalence of class 1 integrons associated antibiotic re- sistance among E. coli strains isolated from different animal sources.

Methods: E. coli strains isolated from different animal sources including human, hen, cow and sheep were included in this study. Bacterial strains were isolated and identified by standard microbiological and biochemical tests. The antimi- crobial susceptibility testing was performed according to Kir- by Baur assay using 11 antibiotic discs including Amikacin, Co-trimoxazole, Tobramycin, Stereptomycin, Piperacillin, Ampicillin, Cefazolin, Nalidixic acid, Gentamicin, Kanamy- cin, and Neomycin. Total genomic and plasmid DNAs were extracted using AccuPrep® Genomic DNA Extraction Kit. To detect the class 1 integron, a newly designed int1 specific primer was used for amplification of integrase gene by PCR. The PCR amplicons were visualized after electrophoresis and staining with SYBR green.

Results: Eighty E. coli strains were isolated and included in this study. Antibiotic susceptibility testing showed that 59% of the isolates were MDR while 31% harbored the int1gene. …

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