Academic journal article Iranian Journal of Public Health

Prevalence of Ctx-3 (Ctx-M 3,15,22) Family Gene in Various E.coli and Klebsiella Pneumoniae Clinical Specimes in Tabriz

Academic journal article Iranian Journal of Public Health

Prevalence of Ctx-3 (Ctx-M 3,15,22) Family Gene in Various E.coli and Klebsiella Pneumoniae Clinical Specimes in Tabriz

Article excerpt

Background: Beta-lactam antibiotics are the most frequently prescribed antibiotics. The emergence of resistance to these antibiotics among gram-negative bacilles has limited their efficiency. This study was done to determine the frequency of producing ESBLs, pattern of resistance and presence of CTX-M3 family gene that contain CTX-M 3,15,22 subfamily in E. coli and Klebsiella pneumoniae isolated from different spec- imen in Sina Hospital of Tabriz

Methods: This study was done on 71 strains of E. coli and 63 strains of K.pneumoniae isolated from Microbiology labora- tory of Sina Hospital in Tabriz, Iran. Bacteria were identified by conventional biochemical and phenothipic testes. Suscep- tibility testing on Muller Hinton was performed with agar disk diffusion method. ESBL producing phinamina in E. coli and K.pneumoniae was detected with combine disc method in our study. Finally the presence of CTX-M3 gene was de- tected by PCR technique and the CTX-M3 family genes were conformed by sequencing.

Results: 41 (57.74%) of E. coli and 45 (71.42%) of K.pneumoniae isolates were ESBL producing. Among the ESBL- prodiucing E. coli 30 (73.17%) and among the ESBL - prodiucing K.pneumoniae 26 (57.77%) of CTX-M3 gene was positive. Antibiotic resistance in E. …

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