Academic journal article Iranian Journal of Public Health

The Study of Qnrs Associated Antibiotic Resistance among Escherichia Coli Strains Isolated from Different Water Sources in Alborz Province

Academic journal article Iranian Journal of Public Health

The Study of Qnrs Associated Antibiotic Resistance among Escherichia Coli Strains Isolated from Different Water Sources in Alborz Province

Article excerpt

Background: Escherichia coli is a frequent cause of life threatening bloodstream and other common infections such as urinary tract infections, while water can be a great source of bacterial transmission. Antibiotic resistance rates especially against fluoroquinolones in E. coli are rapidly rising. Antibiotic- resistant bacteria such as E. coli released from humans and animals into water sources may act as donors of antimicrobial resistance genes to other pathogenic E. coli. The aim of this study was to investigate the prevalence of qnrS associated antibiotic resistance among E. coli strains isolated from different water sources in Alborz province.

Methods: The study included all E. coli strains isolated from different surface water sources in Alborz province in 2013. Bacterial strains were isolated, detected and identified by standard microbiological tests. The antimicrobial susceptibility testing was done according to Kirby Baur assay. Total genomic and plasmid DNAs were extracted by boiling method. All Nalidixic acid and ciprofloxacin resistant strains were examined for the presence of qnrS genes by PCR. The PCR amplicons were visualized after electrophoresis and stained with ethidium bromide. …

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