Academic journal article Iranian Journal of Public Health

Detection of Imipenem Resistance Due to Production of Metallo-Beta-Lactamases in Enterobacteriaceae Isolated from Clinical Specimens by Phenotyping and Genotyping Methods

Academic journal article Iranian Journal of Public Health

Detection of Imipenem Resistance Due to Production of Metallo-Beta-Lactamases in Enterobacteriaceae Isolated from Clinical Specimens by Phenotyping and Genotyping Methods

Article excerpt

Background: Since the incidence of infections caused by MBLs producing Enterobacteriaceae is increasing worldwide, the aim of this descriptive study was the detection of MBLs producing in Enterobacteriaceae strains isolated from clinical specimens in Yazd teaching Hospitals using PCR method.

Methods: This research was performed on Enterobacteriaceae strains collected from various specimens of in-patients in three teaching hospitals dependent to Shahid Sadoughi university medical sciences from 2011.2.1 until 2012.2.19 in Yazd. All isolates were identified by conventional methods. The antimicrobial susceptibility test was performed by Kirby- Bauer method according to CLSI protocols. MBL production was detected by Hodge test. PCR method using specific primers for blaIMP-1, blaVIM-1 and blaNDM was performed.

Results: According to the results, of the 100 Enterobacteriaceae isolated, 26% were resistant to imipenem. Evaluation of Hodge test showed that 7 strains (7%) had Metalo-beta- Lactamases enzyme. 6 of these Metallo-beta-lactamases producing strains was isolated from urine culture and one Metallo- beta-lactamases producing strain was isolated from blood culture. …

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