Academic journal article Iranian Journal of Public Health

Evaluation of the High Level Resistance to the Aminoglycoside in Enterococcus Faecalis by the Multiplex-Pcr Method

Academic journal article Iranian Journal of Public Health

Evaluation of the High Level Resistance to the Aminoglycoside in Enterococcus Faecalis by the Multiplex-Pcr Method

Article excerpt

Background: High-level gentamicin resistance (MIC ≥ 500 mg/L) is commonly intermediated by the aac (6')-Ie-aph (2 '')-Ia gene, which codes the bifunctional enzyme AAC (6 ')- APH (2 ''). In the recent years, three new aminoglycoside resistance genes [aph (2'')-Ib, aph (2'')-Ic and aph (2'')-Id] that also mediate resistance to gentamicin, have been detect- ed in enterococci. Other genes such as the aph (3 ')-IIIa and ant (4')-Ia, that encode the APH (3') and the ANT (4') have also been identified.

Methods: In this study, a total of 100 samples were obtained from the various specimens, including; urine, wound, spu- tum, abscess and tissue. The grown colonies were initially identified by biochemical routine tests. Finally, Multiplex- PCR assay for aac(6 ')-Ie -aph(2'')-Ia, aph(2'')-Ib, aph(2')-Ic, aph(2')-Id, aph(3 ')-IIIa, ant(4 ')-Ia genes amplification were done in order to confirm bacterial colonies as Enterococcus faecalis.

Results: In all, eighty four (84%) Enterococcus faecalis isolates were recovered from 100 specimens. The highest and lowest isolates were related to urine (48%) and sputum (2%), respec- tively. Antibiotic susceptibility test results showed that the highest and lowest resistance is related to the tetracycline and nitrofurantoin, respectively. …

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