Academic journal article Iranian Journal of Public Health

Frequency of Extended-Spectrum Beta-Lactamase- Producing Klebsiella Pneumoniae Isolates from Urinary Tract Infections in Teaching Hospital in Shahrekord by Pcr Method

Academic journal article Iranian Journal of Public Health

Frequency of Extended-Spectrum Beta-Lactamase- Producing Klebsiella Pneumoniae Isolates from Urinary Tract Infections in Teaching Hospital in Shahrekord by Pcr Method

Article excerpt

Background: The aim of this study was to investigate the frequency of ESBL producing Klebsiella pneumonia at an educa- tional hospital in shahrekord, Iran.

Methods: This study was conducted at Shahrekord Universi- ty of medical science. Totally, 150 isolates of Klebsiella pneumo- nia bacteria were selected from out-patient of Hajar and kashani university hospitals. Uropathogens were identified through culture, microscopy and biochemical tests. To detect possible ESBL production, combined double disc synergy test was performed by disc of ceftazidime (30 mg) alone and in the presence of clavulanate (30 mg/10 mg) at a distance of 25 mm, on a Mueller-Hinton agar plate. Detection of SHV gene was examined in ESBL positive strains by PCR.

Results: Combined double disc synergy test was applied to detect ESBL in 75Klebsiella pneumoniae isolates that are re- sistant to ceftazidime using ceftazidime. Among the 48 ESBL-producing K. pneumonia strains, 18(37. …

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