Academic journal article Iranian Journal of Public Health

PREVALENCE OF PLASMID-MEDIATED AMPC B-LACTAMASES: FIRST REPORT OF CMY-2-TYPE AMPC B-LACTAMASE RESISTANCE IN Iran

Academic journal article Iranian Journal of Public Health

PREVALENCE OF PLASMID-MEDIATED AMPC B-LACTAMASES: FIRST REPORT OF CMY-2-TYPE AMPC B-LACTAMASE RESISTANCE IN Iran

Article excerpt

Background: The aim of this study was to determine the prevalence of K. pneumoniae encoded bla CMY-2 gene, isolat- ed from clinical specimens.

Methods: In this Analytic-descriptive cross-sectional study, 144 isolates of Klebsiella spp. were collected from the clinical specimens such as wounds (7cases), supra pubic (1 case), blood (8 cases), septum (2 cases), catheter tips (3 cases), urine (106 cases), CSF (1 case), skin lesion (1 case), tracheal (9 cas- es), anal (2 cases), throat (1 cases) and eye culture (3 case) from Rasht hospitals from February to August 2013. After identification of isolates by biochemical methods, the antibi- otic susceptibility test (Kirby-Bauer method) was done ac- cording to CLSI guideline against 20 antibiotics. The com- bined disk method (Double disk) was then carried out for detection of ESBLs of Klebsiella spp. Among ESBL producers of Klebsiella spp. blaCMY-2 was detected by PCR using spe- cific primers. Then, PCR products were subjected to electro- phoresis on 1.5 % agarose gel. Finally, PCR products were confirmed by sequencing.

Results: Among the Klebsiella 144 clinical isolates, 57 (39. …

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