Academic journal article Iranian Journal of Public Health

Frequency of Methicillin-Resistance among Clinical Isolates of Staphylococcus Aureus by Phenotypic and Molecular Methods in Yazd City

Academic journal article Iranian Journal of Public Health

Frequency of Methicillin-Resistance among Clinical Isolates of Staphylococcus Aureus by Phenotypic and Molecular Methods in Yazd City

Article excerpt

Background: The aim of this study was the survey of fre- quency of methicillin-resistance among clinical isolates of S.aureus by phenotypic and PCR methods in Yazd, Iran.

Methods: In this descriptive-cross sectional study, a total of 114 S. aureus isolates were collected from different specimens of patients admitted to Shahid Sadughi hospital in Yazd, Iran. Antimicrobial susceptibility testing was determined by disk diffusion method (Kirby-Bauer) and minimum inhibitory concentration of oxacillin (MIC) was performed by E.test method. PCR method was performed for detection of the mecA gene using specific primers.

Results: Out of 114 S.aureus strains, 54strains (47.4%) were isolated from wound, 23strains (20.2%) from tracheal aspi- rates, 18 (15.8%) from blood and 12(10.5%) from urine. Sus- ceptibility testing by disk diffusion method was showed that 43 (37.7%) and 49 samples (43%) were resistant to oxacillin and cefoxitin respectively. MIC results showed that 46 (40.5%) samples were resistant to oxacillin, of which 27 (23. …

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