Academic journal article Iranian Journal of Public Health

Pathotypic Comparison of Urinary and Fecal Escherichiacoli Isolated from Children with Urinary Tract Infection

Academic journal article Iranian Journal of Public Health

Pathotypic Comparison of Urinary and Fecal Escherichiacoli Isolated from Children with Urinary Tract Infection

Article excerpt

Background: Uropathogenic Escherichiacoli (UPEC) is a causative agent in most urinary tract infections (UTIs), ex- pressing a multitude of virulence factors. The aim of this study was to investigate the phylogenetic background and the presence of the hlyD and Integron I (intI1) genes in E. coli isolates derived from urinary and fecal specimens.

Methods: A total of 100 E. coli isolates were collected from patients suffering from Urinary Tract Infection (UTI) during September 2009 to September 2010 and screened for hlyD and intI1 genes by polymerase chain reaction (PCR).

Results: Phylogenetic analysis showed that E. coli is com- posed of four main phylogenetic groups (A, B1, B2 and D) and that Uropathogenic E. coli (UPEC) isolates mainly belong to groups B2 (54%) and D (34%) whereas groupA (44%) and D (26%) are predominant in Commensal E. coli isolates. It was shown that hlyD was present in 26% of UPEC and 2% of commensal E. coli isolates, however, hemolysin was ex- pressed by 42% of UPEC and 6% of commensal E. coli iso- lates (p < 0. 05). IntI1 gene was more frequently expressed in UPEC (24%) in comparison with commensal E. coli isolates (12%). …

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