Academic journal article Iranian Journal of Public Health

Prevalence of Some Virulence Genes among Uropathogenic Escherichia Coli Isolates

Academic journal article Iranian Journal of Public Health

Prevalence of Some Virulence Genes among Uropathogenic Escherichia Coli Isolates

Article excerpt

Background: E. coli strains isolated from urinary tract are known as uropathogenic Escherichia coli (UPEC), which cause 80-90% of community acquired urinary tract infection (UTI). UTI usually begins by colonization of pathogenic bacteria in bladder and subsequently the bacterial infection may ascend to kidneys. This study was conducted to investigate some of the virulence factors including fimH, hlyD and tsh, involving in UTI infection among UPEC isolates.

Methods: A total of 85 UPEC isolates from urine samples of outpatients were confirmed by conventional biochemical tests. PCR was done to detect fimH, hlyD and tsh genes us- ing specific pairs of primers for each gene.

Results: PCR assays indicated that adhesins encoding gene, fimH was detected in 29 (34.1%) of the UPEC isolates while toxin encoding the gene, hlyD, and the gene encoding tem- perature sensitive hemagglutinin, tsh, were detected in 18 (21.2%) and 23 (27.1%) of these isolates, respectively. It is noteworthy to mention that 38 (44. …

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