Academic journal article Iranian Journal of Public Health

Distribution of the Subtilase Cytotoxin Gene (Suba) among Shiga Toxin-Producing Escherichia Coli Isolated from Different Sources

Academic journal article Iranian Journal of Public Health

Distribution of the Subtilase Cytotoxin Gene (Suba) among Shiga Toxin-Producing Escherichia Coli Isolated from Different Sources

Article excerpt

Background: Subtilase cytotoxin is a potent AB5 toxin that first discovered in O113: H21 Shiga toxin-producing Esche- richia coli (STEC) strain involving in an outbreak of hemolytic uremic syndrome (HUS) in Australia and introduced in 2004 as a novel bacterial toxin. It has been proposed that this toxin may augment the effects of Shiga toxins and may increase the severity of related diseases in humans. The aim of the present study was to investigate the occurrence of Subtilase encoding gene (subA) among STEC isolates from different sources in Iran.

Methods: For this purpose, a total number of 53 STEC strains that were isolated from cattle (35), sheep (3), pigeon (12) and humans (3) in recent years investigated using a spe- cific polymerase chain reaction (PCR) assay for the subA gene. Then, all STEC isolates were screened for the Stx sub- types using a multiplex-PCR assay for stx1 and stx2.

Results: A total of 21 STEC isolates (39.6%) were shown to harbor the subA gene. …

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