Academic journal article Iranian Journal of Public Health

SURVEY OF COEXISTENCE OF ESBLS WITH AMPC-B-LACTAMASES IN E.COLI IN Iran

Academic journal article Iranian Journal of Public Health

SURVEY OF COEXISTENCE OF ESBLS WITH AMPC-B-LACTAMASES IN E.COLI IN Iran

Article excerpt

Background: The aim of this study was to determine the frequency of the coexistence of ESBLs with AmpC-β- lactamases in E. coli strains as a serious threat for treating bacterial infections.

Methods: During February to August 2013, 288 E. coli strains were isolated from clinical specimens in Rasht hospi- tals. After identification of isolates by biochemical standard methods, the antibiotic susceptibility test (Kirby-Bauer meth- od) was performed according to CLSI standards against 20 antibiotics. The combined disk method (Double disk) was then carried out for detection of ESBLs producers E.coli.The blaCMY-2 gene was detected by PCR method using specific primers among ESBL positive strains. Then, PCR products were subjected to electrophoresis on 1.5 % agarose gel con- taining sybr safe. Finally, sequencing analysis confirmed PCR products as blaCMY-2 gene.

Results: A total of 288 clinical isolates, 118 (41%) were ESBL producers. The most prevalent ESBL producers were isolated from urine sample (102/118). E. coli strains showed the most resistance to Oxacillin (100%) followed by Ampicil- lin, Cephalotin and Amoxicillin (all of them 99. …

Search by... Author
Show... All Results Primary Sources Peer-reviewed

Oops!

An unknown error has occurred. Please click the button below to reload the page. If the problem persists, please try again in a little while.