Academic journal article Iranian Journal of Public Health

GENETIC BACKGROUND OF URINARY ESCHERICHIA COLI STRAINS AND FLUOROQUINOLONE RESISTANCE IN SELSELEH AND DELFAN REGION, LORESTAN, Iran

Academic journal article Iranian Journal of Public Health

GENETIC BACKGROUND OF URINARY ESCHERICHIA COLI STRAINS AND FLUOROQUINOLONE RESISTANCE IN SELSELEH AND DELFAN REGION, LORESTAN, Iran

Article excerpt

Background: Phylogenetic background analyses have shown that E. coli strains fall into four main phylogenetic groups (A, B1, B2, and D) and that virulent extra-intestinal strains be- long mainly to group B2; whereas most commensal strains belong to group A and to group B1. Previous studies have found that greater prevalence of biotype B2 was detected in susceptible than in fluoroquinolone-resistant E. coli strains. The aim of this study was to determine the distribution of phylogenetic groups and their association with fluoroquino- lone susceptibility pattern among uropathogenic E. coli (UPEC) isolates in Selseleh and Delfan, Lorestan, Iran.

Methods: A total of 80 UPEC isolates were collected and biochemically identified from various hospitals in Selseleh and Delfan, Lorestan. After DNA extraction, phylogenetic groups were determined by using established multiplex PCR- based assays. Antibiotic susceptibility pattern of isolates was examined by disk diffusion method according to CLSI rec- ommendations.

Results: The examined isolates (n=80) were distributed in phylogroups A (56.25 %), B1 (18.75 %), B2 (16.25 %) and D (8.75 %). Among 35(43.75%) ciprofloxacin (as a fluoroquino- lone) susceptible isolates, the frequencies of groups A, B1, B2 and D were 62. …

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