Academic journal article Iranian Journal of Public Health

THE RELATIONSHIP BETWEEN VIRULENCE GENES AND O-SEROTYPE OF UROPATHOGENIC E. COLI IN ZABOL, Iran

Academic journal article Iranian Journal of Public Health

THE RELATIONSHIP BETWEEN VIRULENCE GENES AND O-SEROTYPE OF UROPATHOGENIC E. COLI IN ZABOL, Iran

Article excerpt

Background: Escherichiacoli strains that cause urinary tract infections possess several genes encoding urovirulent factors. They are classified based on various types of O antigen. For uropathogenic E. coli, the virulence factor profile of each strain is related with their O-serogroups. The aim of this study was to determine the relationship between eight viru- lence genes and twelve O-Serotypes of uropathogenic E. coli.

Methods: In this study, 100 E. coli samples were collected from patients with urinary tract infection from Zabol hospi- tals (Zabol, Iran). After the biochemical tests to confirm E. coli samples, DNA was extracted using boiling method. The identification of virulence factors and O-serogroups were performed by Multiplex PCR method.

Results: This study determined that fimH with 95% and cnf1 with 28% had the lowest and the highest presence rates of virulence genes, respectively. In addition, the presence of hlyA, iroN, iucD, iha, ompT and irp2 virulence genes were 32, 29, 69, 29, 67 and 89%, respectively. …

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