Academic journal article Iranian Journal of Public Health

PHYLOGENIC TYPING OF ESCHERICHIA COLI ISOLATED FROM BROILERS WITH COLLIBACILLOSIS IN TABRIZ, NORTH WEST OF Iran

Academic journal article Iranian Journal of Public Health

PHYLOGENIC TYPING OF ESCHERICHIA COLI ISOLATED FROM BROILERS WITH COLLIBACILLOSIS IN TABRIZ, NORTH WEST OF Iran

Article excerpt

Background: In this study, to know about the phylogeny of Escherichia coli isolated from broilers with collibacillosis in Tabriz, 70 E. coli isolates recovered from broilers with colli- bacillosis were characterized for phylogenetic group (A, B1, B2, D) by multiplex PCR.

Methods: Bacterial isolates. Seventy E. coli isolates were re- covered from broilers with collibacillosis in Tabriz, North West of Iran from different farms and confirmed according to the biochemical standards s described previously (Quinn et al 1994)and DNA extraction. Multiplex PCR for Phylogenic typing of E. coli. The phylogenic type was determined by mul- tiplex PCR (Clermont et al 2000). Each 25 µl reaction con- tained 0.2 µl DNA templates, 1 U Taq DNA polymerase, 3.2 mM from primer (Table 1), 200 µM of each dNTP, 4 µl of 1 x PCR buffer, and 2 mM MgCl2. …

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