Academic journal article Iranian Journal of Public Health

Detection of N-Acyl Homoserine Lactone (Ahl) in Biofilm Producing Uropathogenic Escherichia Coli Isolated from Urinary Tract Infection (Uti) Samples

Academic journal article Iranian Journal of Public Health

Detection of N-Acyl Homoserine Lactone (Ahl) in Biofilm Producing Uropathogenic Escherichia Coli Isolated from Urinary Tract Infection (Uti) Samples

Article excerpt

Background: N-Acyl homoserine (AHL) is the main com- ponent in quorum sensing and play an important role in bio- film formation. Little information is available regarding to AHL in E.coli. The purpose of this investigation was detec- tion of AHL production among biofilm producing uropatho- genic E. coli isolated from hospital in Kerman, Iran.

Methods: 36 uropathogenic E.colistrains were isolated from Afzal poor hospital during 6 months. The identity of bacterial species was confirmed by biochemical test. Biofilm analysis was done by microtiter plate at OD 490 nm. AHL was sepa- rated from cell mass supernatant by liquid-liquid extraction (LLE) and analyzed by colometric method. AHL functional groups were determined by Fourier transform infra- red spectroscopy (FT-IR).

Results: Among 123 urine sample collected from UTI pa- tients 36 were confirmed as E.coli. The biofilm formation assay introduced 11 (30.55%) isolates that exhibited strong biofilm, 16 (44.44%) showed moderate and 9 (25%) demon- strated weak biofilm. Those uropathogenic E. coli with strong biofilm activity (9 isolates) were subjected to AHL detection. …

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