Academic journal article Iranian Journal of Public Health

Determine the Phylogenetic Groups and Acuity in Factors in Escherichia Coli Urinary Tract Infection

Academic journal article Iranian Journal of Public Health

Determine the Phylogenetic Groups and Acuity in Factors in Escherichia Coli Urinary Tract Infection

Article excerpt

Background: More than 80% of E. coli strains causing uri- nary tract infections in all age groups in the community. E. coli strains are divided into four phylogenetic groups.. Out of intestinal pathogenic isolates belonging to groups D and B2 are often the most acuity factors in compared to groups A and B1. The purpose of this study was to determine phyloge- netic groups and the presence of acuity 8 factor (fimH, iucC,ibeA, sfa/foc, neuC, papC, afa, hly) in Escherichia coli was isolated from urine samples.

Methods: Over a quarter of positive cases of urinary tract infections caused by Escherichia coli had been collected. DNA extraction of the seprated isolates was done andthen PCR was performed using specific primers. The PCRswere elec- trophoresed to determine the groups of phylogeny and acuity in the factors. Analysis of the relationship between variables was done using SPSS software.

Results: From total of 60 E. coli bacteria, the highest fre- quency belong to group D (70%), Group A (3. …

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