Academic journal article Iranian Journal of Public Health

Frequency of Erma,b,c Genes in Erythromycin Resistant Enterococci Isolated from Samples of Hospitalized Patients in University Hospitals Qazvin & Tehran Cities

Academic journal article Iranian Journal of Public Health

Frequency of Erma,b,c Genes in Erythromycin Resistant Enterococci Isolated from Samples of Hospitalized Patients in University Hospitals Qazvin & Tehran Cities

Article excerpt

Background: Enterococci are among the most important caus- es of healthcare associated infections, especially urinary tract infections with multiple drug resistance. The main mecha- nism of erythromycin resistance in Enterococci is 23SrRNA methylation, encoded by erythromycin ribosomal methylases (erm) genes. The aim of this study was to assess the frequen- cy of erm A, B, and C genes in erythromycin-resistant Entero- cocci isolated from the clinical samples of inpatients of univer- sity teaching hospitals in Qazvin and Tehran.

Methods: From May 2012 to June 2013, a total of 165 sam- ples of Enterococci were isolated from the clinical samples of inpatients of five teaching hospitals of Qazvin and Tehran. Suspected colonies on Azide Maltose Agar medium were identified by standard bacteriological methods. Susceptibility to erythromycin in Enterococci isolates was performed by agar dilution and disk diffusion methods according to the Clinical and Laboratory Standards Institute (CLSI) guideline. Eryth- romycin resistance genes (ermA, B, and C) were identified by PCR on erythromycin resistant and intermediate Enterococci isolates.

Results: Out of 165 clinical isolates of enterococci, 142 (86%) isolates were Enterococcus faecalis and 23 (13.9%) isolates Enter- ococcus faecium. The results of susceptibility test showed that 158 (% 95) isolates were resistant or with intermediate sensi- tivity to erythromycin. …

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