Academic journal article Iranian Journal of Public Health

Identification and Antimicrobial Resistance of Human Pathogenic Enterococcus Spp. Isolated from River and Coastal Water

Academic journal article Iranian Journal of Public Health

Identification and Antimicrobial Resistance of Human Pathogenic Enterococcus Spp. Isolated from River and Coastal Water

Article excerpt

Background: The objectives of this study were to describe the species distribution and to evaluate the antimicrobial re- sistance of bacteria from the genus Enterococcus, which were isolated from river and coastal water.

Methods: The collected samples were identified to the genus level by Gram staining, catalase test, hydrolysis of bile-esculin and growth in NaCl 6.5% broth. Species identification was performed by PCR. One hundred and sixty-five (165) Entero- cocci isolates were tested for resistance to eight antibiotics. Diameters of zones of inhibition were recorded in millime- tres and interpreted as sensitive or resistant.

Results: The Enterococci were detected in all of the analyzed water samples. In total, 70 Enterococci were isolated from Ba- bolrud River and coastal waters of Babolsar, of these, 40 were isolated from Coastal water and 30 Babolrud river. Four different Enterococcus spp. were confirmed by PCR: E. faecalis, E. faecium, E.gallinarum and E. casseliflavus. The species distribu- tion of isolates was as follows: Enterococcus faecalis (68. …

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