Academic journal article Iranian Journal of Public Health

Antimicrobial Effect of Clove and Thyme Oil and Combinations on Biofilm Producing Enterococcus Faecalis

Academic journal article Iranian Journal of Public Health

Antimicrobial Effect of Clove and Thyme Oil and Combinations on Biofilm Producing Enterococcus Faecalis

Article excerpt

Background: An important virulence factor of Enterococcus faecalisis its ability to form biofilm. The aim of this study was the activity of Syzygium aromaticum (clove) and Thymus vulgar- is (thyme) and combinations together on E.faecalis biofilm formed in root canal.

Methods: Seventy human intact extracted single-rooted teeth with straight root canal randomly divided in to 5 groups: pos- itive control (n=5), negative control (n=5), clove (n=20), thyme (n=20), clove and thyme (n=20). Each tooth was pre- pared with a crown down technique by using protaper rotary instruments. To formation of biofilm, samples were contam- inated with E.faecalis and incubated at 37°C in the sealed tube for 30 days. The SEM scan was performed to visualize the bacterial biofilm formation. The medicament correspond to the group was placed in the root canal. All specimens were incubated in 100% humidity and 37°C for one week. Anti- bacterial activity was assayed against E. …

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