Academic journal article Iranian Journal of Public Health

Prevalence of Genes Encoding Aminoglycoside Modifying Enzymes in High Level Aminoglycoside Resistant Enterococcus Clinical Isolates by Pcr

Academic journal article Iranian Journal of Public Health

Prevalence of Genes Encoding Aminoglycoside Modifying Enzymes in High Level Aminoglycoside Resistant Enterococcus Clinical Isolates by Pcr

Article excerpt

Background: The aim of this study was to determine the prevalence of high-level Gentamicin and Streptomycin re- sistant and mechanism of resistance to these antibiotics in the clinical isolates of Enterococcus spp.

Methods: From November 2011 to August 2013, a total of 195 enterococcal isolates were collected from clinical samples among 10 medical centers in Tehran, Iran. Confirmation of species was done by conventional tests (Arabinose, xylose, manitol, sorbitol and rhamnose fermentation). Anti-microbial susceptibility test was determined with disk diffusion accord- ing to CLSI (2013) guidelines. Detection of aminoglycoside resistance genes were done by PCR method.

Results: The isolates were found to consist of E. faecalis (65.12%), E. faecium (31.7%), E. gallinarum (2.56%) and E. sulitarius (0.51%).HLGR phenotype was detected in 35.51% of E. faecalis and 69.81% of E. faecium isolates. HLSR pheno- type was detected in 40.18% of E. …

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