Academic journal article Iranian Journal of Public Health

Evaluation of Growth and Cell Morphology of Listeria Monocytogenes Ptcc 1297 as Affected by Various Concentrations of Toxic Heavy Metals

Academic journal article Iranian Journal of Public Health

Evaluation of Growth and Cell Morphology of Listeria Monocytogenes Ptcc 1297 as Affected by Various Concentrations of Toxic Heavy Metals

Article excerpt

Background: Heavy metal pollutants can localize and lay dormant, which can have severe effects on the environment. Unlike organic pollutants, heavy metals do not decay and thus pose a different kind of challenge for remediation. Mi- crobial bioremediation is an efficient strategy due to its low cost and efficient alternative to target heavy metals. The ob- jective of this study was to examine the viability and morpho- logical characteristics of Listeria monocytogenes PTCC 1297 en- dured to toxic heavy metals.

Methods: Different concentrations of the heavy metals used in this study were rgBH2, PbO and CdSo4 (0.1%-0.5% wt/vol). All heavy metals were applied to exponential phase cells whereas non-stressed exponential phase cells served as a control and the cells were allowed to grow for 24 h. For evaluating the viability of L. monocytogenes PTCC1297, after inoculation and exposure of cells to selected concentrations of heavy metals, colony count of them was performed. Scan- ning electron microscopy (SEM) was implemented to visual- ize the surface appearance of bacteria after exposing to stress conditions. …

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