Academic journal article Iranian Journal of Public Health

Isolation and Characterization of a New Strain of Achromobacter Sp. from Zebra Snake (Spalerosophis Microlepis)

Academic journal article Iranian Journal of Public Health

Isolation and Characterization of a New Strain of Achromobacter Sp. from Zebra Snake (Spalerosophis Microlepis)

Article excerpt

Background: This is the first report of isolation of Achromo- bacter sp from Zebra snake (Spalerosophis microlepis).

Methods: Isolation was performed using an enrichment of current bacterial media with minor modification. The kidney was cultured in the appropriate medium and incubated over- night at 37°C. After isolation DNA was extracted and PCR was carried out by 16S rRNA bacterial identification primer and PCR product was sequenced by BIONEER Company (Korea). Finally the sequences were compared with the Gen- Bank database using the Basic Local Alignment Search Tool (BLAST) search.

Results: The unknown bacterium isolate was related to ge- nus Achromobacter . They grow on current bacterial media in aerobic and anaerobic conditions after 48 h incubation at 30 °C. They showed semi moisture round colony in culture. They are catalase-positive, oxidase-positive, gram-negative bacillus. …

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