Academic journal article Iranian Journal of Public Health

Isolation and Screening of Inulinase Producing Bacterial Strains from Soil

Academic journal article Iranian Journal of Public Health

Isolation and Screening of Inulinase Producing Bacterial Strains from Soil

Article excerpt

Background: Microbial Inulinases constitute an important class of enzymes that degrade inulin into fructooligosaccha- rides and high-fructose syrup which are widely used in phar- maceutical and food industry. These enzymes are produced by various microorganisms including bacterial strains, fila- mentous fungi and yeasts. The aim of this study was to iso- late and screen wild-type inulinase producing bacterial strains from soil samples of Gorgan district, Golestan province.

Methods: The soil samples were collected from different locations. The primary screening was performed based on hydrolytic zone on a inulin-based medium and Lugolès io- dine solution. Then morphological and biochemical charac- teristics of the isolated bacterial strains with inulinase activity were determined. Additionally, species-specific identification by 16S rDNA sequencing was performed on a few bacterial strains which had more inulinase activity. …

Search by... Author
Show... All Results Primary Sources Peer-reviewed

Oops!

An unknown error has occurred. Please click the button below to reload the page. If the problem persists, please try again in a little while.