Academic journal article Iranian Journal of Public Health

Changes in Diversity of Pseudomonas Spp. during Bioremediation of Polycyclic Aromatic Hydrocarbons

Academic journal article Iranian Journal of Public Health

Changes in Diversity of Pseudomonas Spp. during Bioremediation of Polycyclic Aromatic Hydrocarbons

Article excerpt

Background: Here we studied the changes in diversity of Pseudomonas spp. during bioremediation of PAHs in soil mi- crocosms prepared using soil samples gathered from KhangI- ran, Khorasan Province, Iran.

Methods: The polluted soil microcosm was constructed by addition of phenanthrene, antheracene, pyrene, and fluoran- thene to the soil, each in 50 ppm concentration (M micro- cosm). Apparently non-polluted soil of the region was used as control (C microcosm). The microcosms were amended with nitrogen, phosphate and humidity and PAHs degrada- tion were monitored in monthly intervals over a six months period. Each month total genomic DNA was extracted from soil using a Fast DNA SPIN Kit. Pseudomonas specific nested PCR combined with denaturing gradient gel electrophoresis (DGGE) were used to analyze the Pseudomonas community profiles. PCR product of excised and reamplified bands in DGGE's gel was sequenced and identified using Eztaxon. …

Search by... Author
Show... All Results Primary Sources Peer-reviewed

Oops!

An unknown error has occurred. Please click the button below to reload the page. If the problem persists, please try again in a little while.