Academic journal article Iranian Journal of Public Health

Effect of Hexadecane on Bioremediation Rate of Polycyclic Aromatic Hydrocarbon

Academic journal article Iranian Journal of Public Health

Effect of Hexadecane on Bioremediation Rate of Polycyclic Aromatic Hydrocarbon

Article excerpt

Background: Here we studied the dynamics of the bacterial community, the number of heterotrophs and PAH degraders, during the bioremediation process of PAHs in presence and abcense of hexadecane.

Methods: Three microcosms prepared using soil sample gathered from KhangIran,khorasan Province Iran,The pol- luted soil microcosm was constructed by addition of phenan- therene, antheracene, pyrene and fluoranthene to the soil, each in 50 ppm concentration with 1% hexadecane (MH microcosm) and the other one without hexadecane (M mi- crocosm). Apparently non-polluted soil of the region was used as control (C microcosm). The microcosms were amended with additional nitrogen and phosphate sources. Humidity and PAHs degradation were monitored in monthly intervals over a six months period. PAHs and hexadecane degradation was monitored by HPLC and GC respectively and the bacterial number assessed by plate count method.

Results: At the first point the number of bacteria is almost the same in all of the three microcosms, but after 8 weeks the number of bacteria in the MH microcosm (1. …

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