Academic journal article Iranian Journal of Public Health

Isolation and Molecular Identification of L-Asparaginase Producing Bacteria from Persian Gulf

Academic journal article Iranian Journal of Public Health

Isolation and Molecular Identification of L-Asparaginase Producing Bacteria from Persian Gulf

Article excerpt

Background: L-asparaginase is an anti-neoplastic agent used in the chemotherapy of lymphoblastic leukemia. Nowadays, this enzyme is derived from bacterial sources. The objective of this study was isolation and molecular identification of L- asparaginase producing bacteria from marine environment in Persian Gulf.

Methods: 53 marine bacteria were isolated from sediment and seawater samples obtained from Persian Gulf in Hor- mozgan, Iran. The isolated strains were screened for L- asparaginase activity. Production of L-asparaginase was car- ried out in medium M9. Based on the enzyme activity among 20 strains producing L-asparaginase, 4 isolates with high L- asparaginase activity were selected and identified base on nucleotide sequence of 16S rRNA gene.

Results: 4 bacteria isolated were identified as Pseudomonas strains and four strains of Bacillus sp. andPseudomonas sp.(PG01) showed the highest productivity of 1.6 IU, and Bacillus sp. (PG04) showed least productivity of 0.67 IU.

Conclusion: This study revealed that bacteria isolated from Persian Gulf may be potential source of enzyme L- asparaginase and Pseudomonas sp. …

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