Academic journal article Iranian Journal of Public Health

Isolation and Identification of Rain-Making Bacteria

Academic journal article Iranian Journal of Public Health

Isolation and Identification of Rain-Making Bacteria

Article excerpt

Background: Ice nucleation active (INA) bacteria have play a role in atmospheric processes and participate in assort of biological precipitation cycle. The most surveys on these bac- teria were from subtropical area, so, studies of INA bacteria from other area are needed. The aim of current research was isolation and identification of INA bacteria from north of Tehran, Iran to investigate their ice nucleation property.

Methods: Bacterial genus were isolated from plant surface (Japan allspice) and winter precipitation (snow and rain) after culturing on BHI medium and incubated in a various tem- peratures (4, 10, 15, 20 and 25°C) for 24-96 hours. Droplet freezing method was used to screen potential of ice nuclea- tion producing bacteria. 16S rRNA analysis was applied to confirm genus and species of the isolates. Outer membrane protein of ice nucleation positive strain was purified and identified by SDS-PAGE method. …

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