Academic journal article Iranian Journal of Public Health

The Prevalence of Escherichia Coli Strains Harboring Ipah Virulence Gene Isolated from Surface Water Sources of Tehran Province

Academic journal article Iranian Journal of Public Health

The Prevalence of Escherichia Coli Strains Harboring Ipah Virulence Gene Isolated from Surface Water Sources of Tehran Province

Article excerpt

Background: E. coli is considered as one of the most im- portant causes of bacterial infections transmitted through water and foods. Several factors are involved in the patho- genesis of E. coli including ipaH gene related to entroinvasive pathotype. The aim of this study was to investigate the preva- lence of E. coli strains carrying virulence gene ipaH isolated from different surface water sources in Alborz province.

Methods: This study was carried out in 2013. The research included all E. coli strains isolated from different surface wa- ter sources of Tehran province including input of 5 water treatment centers. E. coli isolates were detected and identified by standard microbiological and biochemical tests. The strains were evaluated for presence of virulence gene ipaH by PCR using specific primers. The PCR amplicons were visual- ized after electrophoresis and staining with SYBR green. …

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