Academic journal article Iranian Journal of Public Health

Enrichment, Isolation and Identification of Mtbe- Degrading Bacterial from Wastewater Mtbe Plant in Mahshahr

Academic journal article Iranian Journal of Public Health

Enrichment, Isolation and Identification of Mtbe- Degrading Bacterial from Wastewater Mtbe Plant in Mahshahr

Article excerpt

Background: The aim of this work was to isolate and identi- fy soil microorganism capable of degrading MTBE.

Methods: Samples collected from a wastewater MTBE plant of Mahshahr Petrochemical, Iran, and carried in screw-cap bottle to laboratory. Enrichment technique was used to iso- late MTBE degrader. In enrichment studies, a Basal Salts Medium (BSM) was utilized which supplemented with MTBE compound in 6 concentration 50-800 ppm as sole source of carbon and energy. Contaminated soils also used as inoculums in enrichment steps.

Results: According to this research, the consortium obtained from the wastewater of MTBE plant was performed by peri- odically adding MTBE over 3-month period. After several enrichment steps in BSM liquid medium containing MTBE as the sole carbon, of 800ppm Concentration MTBE, a green- blue pigmented strain pw3, (i.e. Pseudomonas aeruginosa) which showed faster rate of MTBE removal, was isolated. It is worth nothing that this bacterium was capable to grow in MTBE concentration of 600ppm as sole carbon and energy source. …

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