Academic journal article Iranian Journal of Public Health

Isolation and Characterization of Methane- Oxidizing Bacteria from Soil

Academic journal article Iranian Journal of Public Health

Isolation and Characterization of Methane- Oxidizing Bacteria from Soil

Article excerpt

Background: The main purpose of current study is isolation and characterization of the bacteria which are capable of me- thane consumptionas the sole source carbon and energy.

Methods: Soil samples were collected from the oil contami- nated regions. The bacterial suspension was prepared and inoculated into NMS (Nitrate mineral salts) broth medium. Then atmosphere of the flasks werefilled up with 50% of methane and 50% of air.Incubation was performed on the shaker for 10 days in30°C in the dark condition. In addition, the gas mixture was renewed every 2 days. Then liquid cul- ture samples werespread onto NMS agar plates. The plates were incubated for 2 weeks in a gas-tight jar with similar gas composition as mentioned above. Single colonies thatformed on the NMS agar plates were transferred onto fresh NMS agar plates and then incubatedfor another week. The contam- ination of samples with non-methane oxidizing bacteria was investigated by incubation of control plates in the absence of methane.Identificationof bacteria was carried out using bio- chemical tests and molecular procedure.

Results: In this research, 2 bacterial strains were isolated from the oil contaminated soils. …

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