Academic journal article Iranian Journal of Public Health

Isolation and Characterization of Antibiotic Producer Actinomycetes Species from the Persian Gulf

Academic journal article Iranian Journal of Public Health

Isolation and Characterization of Antibiotic Producer Actinomycetes Species from the Persian Gulf

Article excerpt

Background: To evaluate the antimicrobial activity of acti- nomycetes isolated from the Persian Gulf, as untapped source for searching new natural antibiotics.

Methods: Initially, water and sediment samples were collect- ed from 23 study sites in the some northwest region of Per- sian Gulf. Isolation was done using Starch casein agar and ISP2 agar (Yeast extract - malt extract agar). Inhibition ef- fects of their extracellular broth were assessed against 5 Gram positive and 4 Gram negative bacteria by top agar layer method. Also pure colonies of bacteria isolates were inocu- lated into ISP2 broth medium and were incubated on a rota- tory shaker (200 rpm) at 28°C for 7-10 days to produce sec- ondary metabolites. Then supernatant was extracted using the solvents such as ethyl acetate, methanol, chloroform, acetone and etc. The optimized solvent was ethyl acetate. Bioactivity of their ethyl acetate extract was assessed at 100 mg/ml concentration by disk diffusion method. Synthetic antibiotics were used as control. Characterization of the best producers was carried out by morphological, physiological, chemotaxonomic and biochemical methods.

Results: 35 strains of actinomycetes were isolated from sam- ples. 18 isolates showed antibacterial activity against at least one of the bacteria tested with more than 15mm diameter of inhibition zone and 5 isolated had more than 25mm diameter of inhibition zone. …

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