Academic journal article Iranian Journal of Public Health

A COMPARISON AMONG VARIOUS SELECTIVE MEDIA TO ISOLATE THE NOVEL HEAT RESISTANT ACTINOMYCETES FROM SOIL SAMPLES OF GANDOMBERIAN AREA OF THE LUT DESERT, Iran

Academic journal article Iranian Journal of Public Health

A COMPARISON AMONG VARIOUS SELECTIVE MEDIA TO ISOLATE THE NOVEL HEAT RESISTANT ACTINOMYCETES FROM SOIL SAMPLES OF GANDOMBERIAN AREA OF THE LUT DESERT, Iran

Article excerpt

Background: The aim of this study was to evaluate different selective media in the isolation of the heat resistant actino- mycetes.

Methods: 46 samples were collected from the surface (sur- face to 5cm depth) and depth (5-20cm depth) soils of three identified places of Gandomberian (30.34N, 57.51E) in the Lut desert, Iran. The soil sample was mixed, and a suspen- sion of 50 g soil in 50 ml of sterile saline solution was pre- pared. To selectively isolate the actinomycetes, the suspen- sion heated in water bath at 55°C for 6 minutes in order to destroy the vegetative forms of bacteria. Four selective cul- ture media (humic acid- vitamin agar containing 50µg/ml cyclohexamide and 10µg/ml nalidixic acid, starch- casein agar containing 25µg/ml cyclohexamide and 25µg/ml nystatin, raffinose- histidine agar containing 25µg/ml cyclohexamide and 25µg/ml nystatin and glucose- yeast extract agar which 20µg/ml rifampicin has been added) were employed. After spreading the samples on the surface of the media, the plates were incubated at 28°C for three weeks, all of the target or- ganisms were counted as actinomycetes and the results ex- pressed as the number of CFU per gram of soil. …

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