Academic journal article Iranian Journal of Public Health

Evaluation and Selection of Additives for Bioremediation of Petroleum-Contaminated Soil Using Oil-Degrading Bacteria

Academic journal article Iranian Journal of Public Health

Evaluation and Selection of Additives for Bioremediation of Petroleum-Contaminated Soil Using Oil-Degrading Bacteria

Article excerpt

Background: As the release of petroleum hydrocarbons into the environment cause harmful effects to ecosystem and hu- man life, treating this contamination by applying sophisticat- ed and economical methods such as bio-remediation is nec- essary and important.

Methods: In this study, additives including bulking agent, nitrogen and phosphorus source were evaluated to find the appropriate additive in order to optimize the bioremediation process in soil contaminated with 5% crude oil which is per- formed by KE1, K-2-4, MK-1, PM01 and PM06 oil degrad- ing bacterial strains isolated from oil contaminated soils from Kharg Island and the maroon region in Ahvaz. This study was conducted in laboratory and pilot scales. The design of experiments (DOE) was done in Minitab 16. Software by Tagouchi methods and orthogonal array of L18 was de- signed. In this study, Straw, Rice husk, Diatomaceous earth, Corn cob, Saw dust, Corn shank were selected 5%(W/W) as the bulking agent, NH4CL, Urea, Uric Acid as the nitrogen source and K2HPO4, KH2PO4 and Lecithin as the phos- phorous with optimum ratio of C: N: P = 100: 10: 1.

Results: Results revealed that straw for PM06 and K-2-4 strains, corn cob for PM01 and MK-1 strains and diatoma- ceous earth for KE1 strain were the appropriate bulking agents to provide optimum conditions of bioremediation. …

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