Academic journal article Iranian Journal of Public Health

Isolation and Identification of Environmental Halophilic Bacteria Producing Industrially Important Enzymes from Salty Soils

Academic journal article Iranian Journal of Public Health

Isolation and Identification of Environmental Halophilic Bacteria Producing Industrially Important Enzymes from Salty Soils

Article excerpt

Background: Industrially important enzymes are endowed with catalytic power to sustain microbial metabolic and phys- iological processes under salt conditions. The aim of the study was isolation and derivation of halophilic bacteria pro- ducing enzymes of industrial value.

Methods: One gram of the collected salty soil samples was weighed and dissolved in 9 ml of sterile distilled water. Serial dilution was done up to 10-5 and cultured on a semi- differential medium supplemented with 10% NaCl using pour plate method and incubated at 30 °C for 72 h. The pure culture of the bacterial isolates was obtained via sub- culturing. To evaluate the activity of amylases, proteases and chitinases, isolates were inoculated onto NA medium sup- plemented with starch 2 % (w/v), skim milk 1 % (w/v) and colloidal chitin 1 % (w/v) respectively. The colonies showing a clear or dim halo were picked as positive exoenzyme- producing isolates. The identity of isolates were studied mor- phologically, biochemically and molecularly. Molecular identi- fication of the selected strains was done by amplification, sequencing and NCBI-BLAST comparison of 16S rDNA sequences employing universal primers 27F (AGAGTTT- GATCCTGGCTCAG) and 1492R (ACGGCTACCTT- GTTACGACTT). …

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