Academic journal article Iranian Journal of Public Health

Bacterial Examination of Cookie and French Fries Boxes

Academic journal article Iranian Journal of Public Health

Bacterial Examination of Cookie and French Fries Boxes

Article excerpt

Background: The aim of this study was to investigate the bacteriological contamination of paper and paperboard, that are used for food packaging. In this way, two testing methods were recruited in order to estimate the number of bacteria.

Methods: Two methods employed including; Agar flooding and smear methods. In the first method, samples of 1×1 cm2 of packaging material were directed to the bottom of the petri dishes and the nutrient medium Tryptone Glucose Ex- tract Agar (TGEA) was added by pour plate procedure. For the second method, 20 cm2 of samples surface were washed out by means of a swab wet-ted in Ringer solution. The wet- ted swab was further put into the flask holding Ringer fluid. Then 2 ml of each sample pour onto 2 Petri dishes with TEAG using pour plate procedure. All Petri dishes were in- cubated at 37 ± 1 °C for 72 hours.

Results: The number of bacteria was enumerated using two mentioned methods. …

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