Academic journal article Iranian Journal of Public Health

Bacterial Isolation from Disposable Foil Containers for Food Packaging

Academic journal article Iranian Journal of Public Health

Bacterial Isolation from Disposable Foil Containers for Food Packaging

Article excerpt

Background: In this study, the bioburden of bacteria on foil was investigated for the first time.

Methods: Two methods employed including; Agar flooding and defibering methods. In the first method, 11 cm × 20 cm of foils were prepared (1 g), which 1cm × 1cm of foil were processed for examination. The tested surface was directed to the bottom of the Petri dishes. Incubation was done at 37 °C for 72 hours. After the incubation had finished, the amount of grown colonies of bacteria was counted for each sample. The number of bacteria was calculated per 1 g of tested sam- ple, per of the foil. Also, defibering method used to enumer- ate the isolated bacteria. In brief, 1 g of sample in 10-2 and 10-3 dilution were prepared in Ringer liquid. 2 ml of each dilution was put onto 3 Petri dishes and flooded with nutri- ent medium Tryptone Glucose Extract Agar (TGEA). The petri dishes were incubated at 37 ± 1 °C for 72 hours. …

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