Academic journal article Iranian Journal of Public Health

Anti-Listerial Activity of Microencap-Sulated Nisin in Different Biopolymers Microparticles

Academic journal article Iranian Journal of Public Health

Anti-Listerial Activity of Microencap-Sulated Nisin in Different Biopolymers Microparticles

Article excerpt

Background: The aim of this study was to evaluate the anti- listerial activity of nisin microencapsulated in three polymeric systems including alginate, alginate-resistant starch and algi- nate-high methoxy pectin.

Methods: Nisin loaded microparticles were prepared by a w/o-emulsion external cross-linking procedure with initial nisin concentration of 600 ppm. Antilisterial activity of nisin encapsulated in alginate, alginate-resistant starch and alginate- high methoxy pectin microparticles was detected by agar diffusion assay.Listeria monocytogenes ATCC 19117 was used as a test microorganism. Three wells of 6 mm diameter were punched into the agar on each plate (that seeded by 100µl of an overnight broth culture containing 107-108 CFU/ml of the test organisms) and lyophilised microparticles were load- ed into wells. Plates were incubated at 37 °C for 24 h. The diameter of inhibition zone (mm) was then measured and reported as anti-listerial activity of microparticles containing nisin.

Results: Nisin loaded in alginate, alginate-resistant starch and alginate-high methoxy pectin exhibited anti-listerial activ- ity with inhibition zone of 11, 17 and 13 mm, respectively. …

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