Academic journal article Iranian Journal of Public Health

The Study of Bla-Ctx Associated Antibiotic Resistance among Escherichia Coli Strains Isolated from Different Water Sources in Alborz Province

Academic journal article Iranian Journal of Public Health

The Study of Bla-Ctx Associated Antibiotic Resistance among Escherichia Coli Strains Isolated from Different Water Sources in Alborz Province

Article excerpt

Background: The aim of this study was to investigate the prevalence of bla-CTX associated antibiotic resistance among E. coli strains isolated from different water sources in Alborz province.

Methods: The study included all E. coli strains isolated from different surface water sources in Alborz province in 2013. Bacterial isolates were all detected and identified by standard microbiological and biochemical tests. The antimicrobial sus- ceptibility testing was determined according to Kirby Baur assay. Total genomic and plasmid DNA were extracted by boiling method. Phenotypic assay for bla-CTX was per- formed by synergic disc then all phenotypically positive ESBL-producing strains were examined for the presence of CTX-M genes by PCR. The PCR amplicons were visualized after electrophoresis and staining with ethidium bromide.

Results: One hundred E. coli strains were isolated and in- cluded in the study. Of these, 38 and 52 isolates were re- sistant to Ceftazidime and Cefazolin, respectively. Thirty eight percentages of the strains were phenotypically ESBL positive. CTX-M gene was detected in 32% of phenotypically ESBL positive strains. …

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