Academic journal article Iranian Journal of Public Health

Distribution of Main E. Coli Phylogroups Encoding Ctx-M Extended- Spectrum Beta-Lactamase (Esbls) in Fecal Isolates of Poultry: A Surveillance Study in Five Poultry Houses in Tehran

Academic journal article Iranian Journal of Public Health

Distribution of Main E. Coli Phylogroups Encoding Ctx-M Extended- Spectrum Beta-Lactamase (Esbls) in Fecal Isolates of Poultry: A Surveillance Study in Five Poultry Houses in Tehran

Article excerpt

Background: Association of avian E. coli phylogroups with human diseases and role of these strains in dissemination of resistance genes to human ones are of major health concerns. This study was aimed to investigate phylogroup diversity and prevalence of genes encoding ESBL among the avian fecal E. coli isolates in five different poultry houses.

Methods: A total of 500 avian cloacal swab samples from five different poultry houses were collected over a six month period in 2013 in Tehran, Iran. Antimicrobial susceptibility testing was done against 13 antibiotics from 9 different clas- ses according to the last CLSI guideline. ESBLs phenotypes were detected by using ceftazidime and ceftazidime- clavulanic acid disks. The presence and type of blaTEM, blaSHV, and blaCTX-M were determined by PCR and se- quencing of these isolates. Diversity of phylogroups A, B1, B2 and D were analyzed among the isolates with confirmed ESBLs phenotype by PCR. The plasmid extracts were screened for carriage of β-lactamase genes.

Results: A total of 444 E. coli isolates were obtained from the studied samples (88.8%). Nearly 92.6% displayed MDR phenotype. A frequency of 5.8% (26) was confirmed for the isolates presenting ESBL phenotype, which blaCTX-M, bla TEM and blaSHV were detected in 6, 10, and 5 strains, re- spectively. …

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