Academic journal article Iranian Journal of Public Health

Prevalence of Listeria Species in Raw Milk in East-Azerbaijan Province

Academic journal article Iranian Journal of Public Health

Prevalence of Listeria Species in Raw Milk in East-Azerbaijan Province

Article excerpt

Background: The genus Listeria consists of six species amongst L. monocytogenes and L. ivanovii are pathogenic to hu- mans and cause listeriosis. Listeriosis may be transmitted through direct contact with infected animals or by consump- tion of contaminated raw milk and dairy products. This study aimed to investigate the prevalence of Listeria spp. in bulk raw milks of East-Azerbaijan province.

Methods: A total sample of 85 bulk-raw milks was collected from six distinct parts of East-Azerbaijan province. The samples were analyzed for the presence of Listeria spp. using conventional culture methods. For primary enrichment, 25 ml of each sample was added to 225 ml of Listeria enrichment broth (UVM I) and incubated at 37°C for 24 h. Afterwards, for secondary enrichment 0.1 ml of UVM I was transferred to 9 ml of UVM II and incubated at 37°C for 24 h. Both primary and secondly enrichment broths were streaked on PALCAM Listeria Selective agar and were incubated at 37°C for 24-48 h. Green-gray colonies with beige halo were sub- jected to differential morphological/biochemical analysis including, Gram staining, catalase, MR/VP, motility at 25°C and 37°C, acid production from glucose, manitol, rhamnose, xylose, α-methyl-D-manoside, nitrare reduction, hydrolysis of esculin, β-hemolytic activity and CAMP test. …

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