Academic journal article Iranian Journal of Public Health

The Study of Prevalence of Gene Esta in Escherichia Coli Strains Isolated from Different Surface Water Sources in Alborz Province

Academic journal article Iranian Journal of Public Health

The Study of Prevalence of Gene Esta in Escherichia Coli Strains Isolated from Different Surface Water Sources in Alborz Province

Article excerpt

Background: Escherichia coli is a member of the normal intes- tinal microflora of humans and of many animal. Although most strains of E. coli are harmless but some types of E. coli especially ETEC pathotypes cause intestinal diseases and are transmitted through water and foods. The aim of this study was to investigate the prevalence of E. coli strains carrying virulence gene, encoding enterotoxin (estA) isolated from different water sources in Alborz province.

Methods: This study was carried out in 2013. The research included all E. coli strains isolated from different surface wa- ter sources in Alborz province. E. coli strains were isolated, detected and identified by standard microbiological and bio- chemical tests. The strains were evaluated for presence of virulence gene estA by PCR using specific primers. The PCR amplicons were visualized after electrophoresis and stained with ethidium bromide.

Results: Ninety nine E. coli strains were isolated and includ- ed in the study. Approximately an equal isolation rate of ETEC was seen among different water source. …

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