Academic journal article Iranian Journal of Public Health

Identification of Multi-Drug Resistant Staphylococcus Aureus Isolates in Samples Obtained from Medical Foods, Kitchen Utensils and Food Handlers in a Hospital in Tehran

Academic journal article Iranian Journal of Public Health

Identification of Multi-Drug Resistant Staphylococcus Aureus Isolates in Samples Obtained from Medical Foods, Kitchen Utensils and Food Handlers in a Hospital in Tehran

Article excerpt

Background: Multi- drug resistant (MDR) S. aureus is one of the most important etiological factors in food-borne diseases in predisposed patients at medical centers. This study was carried out to determine the frequency of MDR- S. aureus isolates in samples collected from hospital foods and associa- tion of their drug resistance pattern with those of samples obtained from kitchen utensils and food handlers.

Methods: A total of 144 samples were collected from hospi- tal foods, kitchen utensils and hands of the food handlers during food preparation in 2012-2013. The samples were transported to the microbiology laboratory in order to detect S. aureus contamination. Culture in mannitol salt agar media, morphological evaluation of the grown colonies, specialized tests such as catalase, oxidase, hemolysis, coagulase, DNase, as well as PCR testing (for femA and nucA genes) were done for more identification of the isolates. Drug-resistance pat- tern of the S. aureus isolates were also analyzed with the disk diffusion method in accordance with CLSI, 2012 guidelines.

Results: S.aureus bacteria were isolated in 12 out of 72 food samples (16.7%), 15 out of 18 samples from food handlers (83%) and 24 out of 54 utensil samples (44. …

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